Next Generation Biotic Solutions

Saltose Pro

 

New Probiotics & Strong Enzyme Combination

 

Saltose Pro” is a live bacteria feed for poultry, livestock, and aquaculture that combines several useful bacteria, mainly Bacillus subtilis, B. licheniformis, and B. amyloliquefaciens, and lactic acid bacteria,

with multiple active enzymes.

 

Saltose Pro” is a bio-agent developed for the purpose of creating strong livestock that are resistant to

various bacteria that may infect or remain in poultry, eggs, chicken, pork, beef, milk, etc. 

  

Saltose Pro” incorporates a technology that prevents deterioration of the active capacity of the biocide.

We have succeeded in stabilizing and promoting the activity of the bacteria at a high level.

 

SALTOSE is a solution probiotic feed for infections caused by Clostridium, Salmonella, Coccidiosis, and Cryptosporidium using our proprietary “cell wall degrading enzyme”.

 

Combination of Multi probiotics and powerful Multi enzymes

 

Our Bacillus strains, selected from 10,000 different strains and special enzymes, control harmful bacteria (Salmonella, E. coli, Clostridium) and parasites (Coccidiosis, Cryptosporidium) to ensure the safety and health of poultry and livestock.

 

SunrenBio Inc is engaged in the research and development of high-quality, antibiotic-free functional feed, probiotics, and enzyme products for poultry and livestock, Aquaculture production. One of its product lines, Saltose Pro, has been recognized for its unique functionality in the poultry and livestock industry both in Japan and globally.  

The Bacillus group

>1×10⁹cfu/g

 

1.Bacillus licheniformis

2.Bacillus subtilis

3.Bacillus amyloliquefaciens

 

The main enzymes;

1.Protease

2.Beta-Xylanase

3.Cell-Wall Lyase (Original Lysozyme)

The Enterococcus group

>1×10⁹cfu/g

 

1.Enterococcus faecalis

2.Enterococcus faecium



Lysis Effects on Eggs of Parasites or Oocysts 2 - 1                                                   

 

Coccidium oocyst (Poultry)

Before addition of SALTOSE PRO 

Coccidium oocysts germinated and sporocysts developed.

After addition of SALTOSE PRO 

Coccidium oocyst has completely dissolved, and the original form has disappeared.


Coccidium oocyst (cattle)

Before addition of SALTOSE PRO 

Coccidium oocysts germinated and sporocysts developed.

After addition of SALTOSE PRO

Coccidium oocyst has completely dissolved, and the original form has disappeared.


 Lysis Effects on Eggs of Parasites or Oocysts 2 - 2

 

Cryptosporidium (Cattle)

 

Prepared culture media for SALTOSE and inoculated Cryptosporidium for observation of degradation effects.

Before addition of SALTOSE 

12 hours after addition of SALTOSE

 


Eventually the Cryptosporidium completely dissolved and its original form disappeared.

>Inhibitory Effects on Bacteria 2 - 1

Adjust the culture medium for S. enteritidis and inoculate with the following bacteria

 

1, 6, 7, 8, 9 : SALTOSE PRO Bacteria

T : Competitor

C : Competitor

S1 : S.Typhimurium

S2 : S.Gallinarum

 

SALTOSE exhibits antibacterial activity through the formation of an inhibition circle.

Salmonella Enteritidis and Salmonella Gallinarum

To observe inhibition cycles, RL bacteria were inoculated into YM-SE and YM-SG media.

YM : Yeast and Malt

RL : Sartoze bacteria

SE : S.Enteritidis

SG : S.Gallinarum

SP : Other Bacillus subtilis

SALTOSE produced a large inhibition circle, showing strong control effects against S. Enteritidis and S. Gallinarum.

Other Bacillus subtilis showed little control.

 

 

 


>Inhibitory Effects on Bacteria 2 - 2                                                                     

 

Clostridium 

Clostridium media were prepared and inoculated with SALTOSE Bacillus licheniformis (right side) and other Bacillus licheniformis (left side) bacteria and growth and inhibition circles were observed.

12hours

60 hours

32hours


32 hours after the inoculation, Bacillus licheniformis of SALTOSE stopped their growth due to completing the absorption of nutrients from culture medium.

However, inhibition ring continued to expand, and dissolved Clostridium.

 

 

Antagonistic Effects on Bacteria 3 - 1

Salmonella Enteritidis(840) and Salmonella Infantis (837)

Competitor

Competitor did not absorb the inoculated Salmonella bacteria.

 

SALTOSE

SALTOSE absorbed the inoculated Salmonella bacteria.

 


Antagonistic Effects on Bacteria 3 - 2

Salmonella Gallinarum

S.Gallinarum were inoculated in DP (Left side) and YM (Right side) culture medium, and then, each bacterium was inoculated in the same point for observation of antagonistic effects.

DP : Dexton and Pepton

SE : S.Enteritidis

RL : SALTOSE bacteria

SP : Other Bacillus subtilis

YM : Yeast and Malt

SG : S.Gallinarum

 

 

1.The growth power of SALTOSE bacteria is the strongest, and SALTOSE bacteria controls S.Gallinarum bacteria

2.Other Bacillus subtilis indicated poor growth power.


Antagonistic Effects on Bacteria 3 - 3

E-Coli(K-88)


E. coli (K-88) and SALTOSE bacteria were inoculated on the same culture medium. It is observed that SALTOSE shows more antibacterial activities against E. coli .

 

 

Useful Bacteria 

Lactic acid Bacteria

Bifido Bacteria


SALTOSE did not damage the useful bacteria.

 

 

>The Other Data

Testing result (Excreta of poultry)

1. Reduction of generating ammonium odor

Generation of ammonia from broiler farm was reduced due to application of SALTOSE.

2.Comparison of digestibility

Result: SALTOSE treatment can improve the digestion, so the amount of undigested materials in the excreta becomes half as compared with the control.

2-2.Saltose bacteria support digestion and absorption of feed ingredients.

It can be seen that the soybean meal is more degraded in the test area than in the control area.

This suggests that even currently unused carbohydrates may be utilized as nutrients.

3. Bacterial 1 count in the excretion of chickens

The count of Bacillus bacteria in the excretion remained almost unchanged; however, the total bacteria count was reduced as dosage rate was increased.

4.Enzyme production capacity test of Saltose Pro

Tested by: Institute in Taiwan 

Method: Bacteria were isolated from the live feed of each company, incubated for 48 hours, and the amount of enzyme produced (unit: ug/g) was measured.

5.SaltosePro Heat resistance test