Next Generation Biotic Solutions
The Bacillus group
>１×10⁹cfu/g
1.Bacillus licheniformis
2.Bacillus subtilis
3.Bacillus amyloliquefaciens
The main enzymes;
1.Protease
2.Beta-Xylanase
3.Cell-Wall Lyase (Original Lysozyme)
The Enterococcus group
>１×10⁹cfu/g
1.Enterococcus faecalis
2.Enterococcus faecium
■Lysis Effects on Eggs of Parasites or Oocysts 2 - 1
Coccidium oocyst (Poultry)
Before addition of S PRO
Coccidium oocysts germinated and sporocysts developed.
After addition of SPRO
Coccidium oocyst has completely dissolved, and the original form has disappeared.
Coccidium oocyst (cattle)
Before addition of SPRO
Coccidium oocysts germinated and sporocysts developed.
After addition of SPRO
Coccidium oocyst has completely dissolved, and the original form has disappeared.
■Lysis Effects on Eggs of Parasites or Oocysts 2 - 2
Cryptosporidium (Cattle)
Prepared culture media for SALTOSE and inoculated Cryptosporidium for observation of degradation effects.
Before addition of S
12 hours after addition of S
Eventually the Cryptosporidium completely dissolved and its original form disappeared.
＞Inhibitory Effects on Bacteria 2 - 1
Salmonella Enteritidis and Salmonella Gallinarum
To observe inhibition cycles, RL bacteria were inoculated into YM-SE and YM-SG media.
YM : Yeast and Malt
RL : Sartoze bacteria
SE : S.Enteritidis
SG : S.Gallinarum
SP : Other Bacillus subtilis
S produced a large inhibition circle, showing strong control effects against S. Enteritidis and S. Gallinarum.
Other Bacillus subtilis showed little control.
>Inhibitory Effects on Bacteria 2 - 2
Clostridium
Clostridium media were prepared and inoculated with S Bacillus licheniformis (right side) and other Bacillus licheniformis (left side) bacteria and growth and inhibition circles were observed.
12hours
60 hours
32hours
32 hours after the inoculation, Bacillus licheniformis of S stopped their growth due to completing the absorption of nutrients from culture medium.
However, inhibition ring continued to expand, and dissolved Clostridium.
Antagonistic Effects on Bacteria 3 - 1
Salmonella Enteritidis(840) and Salmonella Infantis (837)
Competitor
Competitor did not absorb the inoculated Salmonella bacteria.
S
S absorbed the inoculated Salmonella bacteria.
Antagonistic Effects on Bacteria 3 - 2
Salmonella Gallinarum
S.Gallinarum were inoculated in DP (Left side) and YM (Right side) culture medium, and then, each bacterium was inoculated in the same point for observation of antagonistic effects.
DP : Dexton and Pepton
SE : S.Enteritidis
RL : S bacteria
SP : Other Bacillus subtilis
YM : Yeast and Malt
SG : S.Gallinarum
1.The growth power of S bacteria is the strongest, and S bacteria controls S.Gallinarum bacteria
2.Other Bacillus subtilis indicated poor growth power.
Antagonistic Effects on Bacteria 3 - 3
E-Coli(K-88)
E. coli (K-88) and S bacteria were inoculated on the same culture medium. It is observed that S shows more antibacterial activities against E. coli .
Useful Bacteria
Lactic acid Bacteria
Bifido Bacteria
S did not damage the useful bacteria.
＞The Other Data
Testing result (Excreta of poultry)
1. Reduction of generating ammonium odor
Generation of ammonia from broiler farm was reduced due to application of S
2.Comparison of digestibility
Result: S treatment can improve the digestion, so the amount of undigested materials in the excreta becomes half as compared with the control.
2-2.S bacteria support digestion and absorption of feed ingredients.
It can be seen that the soybean meal is more degraded in the test area than in the control area.
This suggests that even currently unused carbohydrates may be utilized as nutrients.
3. Bacterial 1 count in the excretion of chickens
The count of Bacillus bacteria in the excretion remained almost unchanged; however, the total bacteria count was reduced as dosage rate was increased.
4.Enzyme production capacity test of S
Tested by: Institute in Taiwan
Method: Bacteria were isolated from the live feed of each company, incubated for 48 hours, and the amount of enzyme produced (unit: ug/g) was measured.